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Then, radiolabeled 125 I-GLP-1 (40 pM, PerkinElmer) and increasing concentrations of unlabeled peptide were added and competitively reacted with the cells in binding buffer (PBS supplemented with 10% (w/v) BSA, pH 7.4) at RT for 3 h

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In addition to being generated during respiration in mitochondria, hydrogen peroxide is also produced from various other metabolic processes in cells, including protein folding

In a laboratory setting, studying the direct application of IGF-1 LR3 allows researchers to observe its potent localized effects on cell proliferation and differentiation, bypassing the systemic release cycle

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